Experimental design: ANOVA? help!

A

Arwen82

Guest
#1
Hello,

I have to analyze one paramater (lipid content) of seeds from 5 different cultivars. I am planning to do two independent analysis of the parameter from the 5 cultivar seeds, with 3 technical replicates for each independent analysis. I will have then 2 independent experiment with 3 technical replicates for every cultivar.

1. Should I analyze the differences in the parameter from the 5 cultivars using ANOVA?

2. Should the "N" be 2? (mean of the 3 replicates for the 2 independent experiments)
or can I pool all the replicates from the 2 independent experiments and to have a N = 6?

Thank you very much.